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Some states like Florida Require Boat tie downs. Do not just rely on the winch strap. You should have 2 in the back and one in the front in addition to the winch strap.
Check the trailer lights. All of them and if not LED, then carry spare bulbs.

The vehicle jack and lug wrench probably will not fit the trailer. Check to make sure you Can change a tire.
Have you ever seen a trailer swerving behind a vehicle on a highway? That's because the tongue weight is not correct.
7-10% is required of the total weight of the boat and trailer on the tongue.

I mounted my trailer lights up high on the 2 left and right guide poles mounted to the trailer. They are easier to see and act like the high brake lights on vehicles.

Tie everything down in the boat that is not fastened to it. Items will blow out and you lose them. Keep the drain plugs out, if it rains, you do not want to trailer a boat full of water.